Analysis methods Print E-mail
CONSTITUENT METHOD (Principle, Reference text) COFRAC accreditations (Programme no.) Quantification limit Number of test samples for analysis
Number of measurements per test sample 
Moisture AFNOR NF V 04-401 (desiccation at 103°C for 16 hours) 80 0,1g/100g 2 1
Ash AFNOR NF V 04-404 80 0,1g/100g 2 1
Protein AFNOR NF V 04-407 80 0,1g/100g 2 1
Total lipids Hydrochloric treatment and hexane extraction then weighed 80 0,1g/100g 2 1
Carbohydrate per calculation        
Energy per calculation        
Calcium Atomic absorption spectrometry, Decree of 08/09/1977, OJ of 03/11/1977, NFV 18-108 60 0,1mg/100g 2 1
Potassium Atomic absorption spectrometry, Decree of 08/09/1977, OJ of 03/11/1977, NFV 18-108 60 0,1mg/100g 2 1
Magnesium Atomic absorption spectrometry, Decree of 08/09/1977, OJ of 03/11/1977, NFV 18-108 60 0,1mg/100g    
Sodium Atomic absorption spectrometry, Decree of 08/09/1977, OJ of 03/11/1977, NFV 18-108 60 0,1mg/100g 2 1
Copper Atomic absorption spectrometry, 8th CEE dir. 78/633 modified by CEE dir. 81/680 and 84/4 60 0,1mg/100g 2 1
Iron Atomic absorption spectrometry, 8th CEE dir. 78/633 modified by CEE dir. 81/680 and 84/4 60 0,1mg/100g 2 1
Manganese Atomic absorption spectrometry, 8th CEE dir. 78/633 modified by CEE dir. 81/680 and 84/4 60 0,1mg/100g 2 1
Zinc Spectrométrie d'absorption atomique, 8ème dir. CEE 78/633 modifiée par dir. CEE 81/680 et 84/4 60 0,1mg/100g 2 1
Iode          
Selenium Atomic absorption spectrometry preceded by hydride generation then mineralisation to a limit lower than the atomic absorption spectrometry limit with Zyman correction preceded by sulphonitric attack and specific   5µg/100g 2 1
Chloride Potentiometry   10mg/100g 2 1
Vitamine A (retinol) High Performance Liquid Chromatography to detect absorption then UV, AFNOR NF EN 12823-1 60 2µg/100g 2 1
Vitamine E High Performance Liquid Chromatography, AFNOR NF EN 12823-2 60 0,01mg/100g 2 1
Vitamine D High Performance Liquid Chromatography   0,5µg/100g 2 1
Vitamine B1 (thiamine) Reverse phase High Performance Liquid Chromatography and detection by fluorometry, modified decree of 12/01/1999 60 0,04mg/100g 2 1
Vitamine B2 (riboflavine) Reverse phase High Performance Liquid Chromatography and detection by fluorometry, modified decree of 12/01/1999 60 0,04mg/100g 2 1
Vitamin B3 or PP (niacine) Microbiological method AOAC 944-13 with test strain Lactobacillus plantarum 60 0,16mg/100g 2 1
Vitamin B5 (pantothenic acid) High Performance Liquid Chromatography Method AOAC 945-74 60 0,16mg/100g 2 1
Vitamine B6 High Performance Liquid Chromatography according to EN 14663 60 0,02mg/100g 2 1
Vitamin B12 (cobalamin) Microbiological method according to AOAC 952-20   0,08µg/100g 2 1
Beta-carotene High Performance Liquid Chromatography according to NF EN 12823-2 60 4µg/100g 2 1
Total carotenoids High Performance Liquid Chromatography   0,01mg/100g 2 1
Amino acid profile High Performance Liquid Chromatography according to directive 98/64 NF V 18-114   20µg/100g 1 1
Cholesterol Determination of individual and total sterol content NF EN ISO 12228 CPG Method   10mg/100g of extracted fat 1 1
Fatty acid profile Determination of extracted fat content using the Folch method
Preparation and analysis by gas phase chromatography of fatty acid methyl esters NF EN ISO 5509et NF EN ISO 5508
82 1g/100g

0.1g/100g of extracted fat
2 1