| CONSTITUENT |
METHOD (Principle, Reference text) |
COFRAC accreditations (Programme no.) |
Quantification limit |
Number of test samples for analysis
|
Number of measurements per test sample
|
| Moisture |
AFNOR NF V 04-401 (desiccation at 103°C for 16 hours) |
80 |
0,1g/100g |
2 |
1 |
| Ash |
AFNOR NF V 04-404 |
80 |
0,1g/100g |
2 |
1 |
| Protein |
AFNOR NF V 04-407 |
80 |
0,1g/100g |
2 |
1 |
| Total lipids |
Hydrochloric treatment and hexane extraction then weighed |
80 |
0,1g/100g |
2 |
1 |
| Carbohydrate |
per calculation |
|
|
|
|
| Energy |
per calculation |
|
|
|
|
| Calcium |
Atomic absorption spectrometry, Decree of 08/09/1977, OJ of 03/11/1977, NFV 18-108 |
60 |
0,1mg/100g |
2 |
1 |
| Potassium |
Atomic absorption spectrometry, Decree of 08/09/1977, OJ of 03/11/1977, NFV 18-108 |
60 |
0,1mg/100g |
2 |
1 |
| Magnesium |
Atomic absorption spectrometry, Decree of 08/09/1977, OJ of 03/11/1977, NFV 18-108 |
60 |
0,1mg/100g |
|
|
| Sodium |
Atomic absorption spectrometry, Decree of 08/09/1977, OJ of 03/11/1977, NFV 18-108 |
60 |
0,1mg/100g |
2 |
1 |
| Copper |
Atomic absorption spectrometry, 8th CEE dir. 78/633 modified by CEE dir. 81/680 and 84/4 |
60 |
0,1mg/100g |
2 |
1 |
| Iron |
Atomic absorption spectrometry, 8th CEE dir. 78/633 modified by CEE dir. 81/680 and 84/4 |
60 |
0,1mg/100g |
2 |
1 |
| Manganese |
Atomic absorption spectrometry, 8th CEE dir. 78/633 modified by CEE dir. 81/680 and 84/4 |
60 |
0,1mg/100g |
2 |
1 |
| Zinc |
Spectrométrie d'absorption atomique, 8ème dir. CEE 78/633 modifiée par dir. CEE 81/680 et 84/4 |
60 |
0,1mg/100g |
2 |
1 |
| Iode |
|
|
|
|
|
| Selenium |
Atomic absorption spectrometry preceded by hydride generation then mineralisation to a limit lower than the atomic absorption spectrometry limit with Zyman correction preceded by sulphonitric attack and specific |
|
5µg/100g |
2 |
1 |
| Chloride |
Potentiometry |
|
10mg/100g |
2 |
1 |
| Vitamine A (retinol) |
High Performance Liquid Chromatography to detect absorption then UV, AFNOR NF EN 12823-1 |
60 |
2µg/100g |
2 |
1 |
| Vitamine E |
High Performance Liquid Chromatography, AFNOR NF EN 12823-2 |
60 |
0,01mg/100g |
2 |
1 |
| Vitamine D |
High Performance Liquid Chromatography |
|
0,5µg/100g |
2 |
1 |
| Vitamine B1 (thiamine) |
Reverse phase High Performance Liquid Chromatography and detection by fluorometry, modified decree of 12/01/1999 |
60 |
0,04mg/100g |
2 |
1 |
| Vitamine B2 (riboflavine) |
Reverse phase High Performance Liquid Chromatography and detection by fluorometry, modified decree of 12/01/1999 |
60 |
0,04mg/100g |
2 |
1 |
| Vitamin B3 or PP (niacine) |
Microbiological method AOAC 944-13 with test strain Lactobacillus plantarum |
60 |
0,16mg/100g |
2 |
1 |
| Vitamin B5 (pantothenic acid) |
High Performance Liquid Chromatography Method AOAC 945-74 |
60 |
0,16mg/100g |
2 |
1 |
| Vitamine B6 |
High Performance Liquid Chromatography according to EN 14663 |
60 |
0,02mg/100g |
2 |
1 |
| Vitamin B12 (cobalamin) |
Microbiological method according to AOAC 952-20 |
|
0,08µg/100g |
2 |
1 |
| Beta-carotene |
High Performance Liquid Chromatography according to NF EN 12823-2 |
60 |
4µg/100g |
2 |
1 |
| Total carotenoids |
High Performance Liquid Chromatography |
|
0,01mg/100g |
2 |
1 |
| Amino acid profile |
High Performance Liquid Chromatography according to directive 98/64 NF V 18-114 |
|
20µg/100g |
1 |
1 |
| Cholesterol |
Determination of individual and total sterol content NF EN ISO 12228 CPG Method |
|
10mg/100g of extracted fat |
1 |
1 |
| Fatty acid profile |
Determination of extracted fat content using the Folch method
Preparation and analysis by gas phase chromatography of fatty acid methyl esters NF EN ISO 5509et NF EN ISO 5508 |
82 |
1g/100g
0.1g/100g of extracted fat |
2 |
1 |
